Influence of different concentrations of enamel matrix proteins on bioactivity of human periodontal ligament cells

doi:10.3969/j.issn.2095-4344.2015.03.013

Abstract

Abstract:

BACKGROUND: Numerous studies have confirmed that enamel matrix proteins can promote the regeneration of osteoblasts and cementoblast, and then it can achieve approaching physiological periodontal regeneration in the treatment of periodontal defects.

OBJECTIVE: To observe the effects of different concentrations of enamel matrix proteins on proliferation, viability, differentiation and migration of human periodontal ligament cells.

METHODS: The human periodontal ligament cells at the third generation were gained, and then cultured in serum-free DMEM containing different concentrations of enamel matrix proteins (0, 12.5, 25, 50, 100, 250 mg/L). After 24 hours of culture, proliferation and viability of periodontal ligament cells were measured using [(3)H]-thymidine uptake and MTT assay. After 48 hours of culture, alkaline phosphatase activity and osteocalcin production were detected with commercial available test kits. When the cells grew as a monolayer, the cell culture fluid was removed, and then with a pipette head, a cell incision, 1 mm wideness, was prepared in a monolayer of cells to further observe the cell fusion continuously within 24 hours.

RESULTS AND CONCLUSION: The proliferation, viability and differentiation of periodontal ligament cells were gradually increased with the concentration increase of enamel matrix proteins (0-100 mg/L). When the concentration of enamel matrix proteins was 250 mg/L, these parameters began to decrease, but the levels were still higher than those in the 0 mg/L enamel matrix protein group. In the 100 mg/L group, the cells in the wound edge began to grow towards the center in the initial 6 hours, then became confluent at 12 hours, and until the 20^th hour of culture, the cells in the two sides of the wound edge were completely fused to fully close the wound edge, indicating a better wound healing in the 100 mg/L group than the other groups. These findings suggest that enamel matrix proteins can stimulate proliferation, viability, differentiation and migration of periodontal ligament cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Periodontal Ligament, Extracellular Matrix Proteins, Cell Movement

CLC Number:

R318

Qu Zhe, Zhang Jing-ying, Guo Ying, Ma Wei-dong, Ma Lan. Influence of different concentrations of enamel matrix proteins on bioactivity of human periodontal ligament cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(3): 399-404.

Figures/Tables 5

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